Figure 10 Overall survival according to BAG-1 expression which was based on platinum chemotherapy (32.3 vs. 15.2 months, P = 0.002). Correlation of ERCC1 and BAG-1 expression There were 25 cases that expressed both ERCC1 and BAG-1 and 27 cases that expressed neither. As shown in Table 5, the buy RG7112 correlation was found between ERCC1 and BAG-1 gene expression (P = 0.042, r = 0.247). All 52 patients of both positive and negative expression were received adjuvant chemotherapy. For both negative mRNA expression had a significantly longer median progression-free (more than 42.6 months vs. 8.8 months, P = 0.000) and overall (more than 42.6 months vs. 17.0 months, P = 0.000) survival, compared
with those positive for both ERCC1 and BAG-1 expression (Figures 11, 12). Table 5 Correlation between expression of ERCC1 and BAG-1 Gene ERCC1 + – + 25 8 BAG-1 – 25 27 Figure 11 Progression-free survival according to 52 NSCLC patients who have both ERCC1 and BAG-1 expression, all of whom were based on platinum chemotherapy (more than 42.6 vs. 8.8 months, P = 0.000). Figure 12 Overall survival according to 52 NSCLC patients AZD1390 in vivo who have both ERCC1
and BAG-1 expression, all of whom were based on platinum chemotherapy (more than 42.6 vs. 17.0 months, P = 0.000). Discussion Along with the development of theory and practice in selleck chemicals llc treatment of chemotherapy with resected NSCLC, we have already known the combination of two cytotoxic drugs, like a platinum and a non-platinum agent, is the standard first-line treatment of NSCLC patients . However, because of the high rate of toxicity observed and associated with drug resistance, treatment response rate and median overall survival are not satisfactory. This appears to be gene of chemoresistance, which plays an important role in the after surgery treatment. So, some markers detection is a key for chemotherapy in NSCLC patients. Platinum drugs mainly exert their cytotoxicity by forming bulky intra-strand
platinum-DNA adducts and inter-strand cross-link of the two DNA strands. Removal of these adducts from genomic DNA and repair of inter-strand cross-links in DNA and recombination processes are mediated by components of different Dapagliflozin DNA repair pathways. ERCC1 is a key factor involved in nuclear excision repair (NER) for platinum induced adducts . There is observation of platinum resistance in lung cancer A549 cells lines with high expression of ERCC1 , and increased clinical evidence that overexpression of ERCC1 in NSCLC inhibits platinum efficacy. In addition to ERCC1 negative tumors appear to benefit from cisplatin based chemotherapy, it also gains benefit from overall survival as a prognostic factor [2, 15, 16]. As a predictive factor, a phase III trial in NSCLC showed better PFS and OS in the low genotypic than in the high genotypic group, and the patients in the low genotypic group also had a trend toward a lower risk of progression than those in the control arm .