The latter two traits and formation of crystals on CMD do not seem consistent, because the H. pachybasioides strain CBS 119319 behaves similarly. The semiglobose warts on elongations of H. parapilulifera may be diagnostic for the species if consistent among the strains. The isolate from the Czech specimen sporulated www.selleckchem.com/products/ch5424802.html only on SNA, while Lu et al. (2004) reported also conidiation on CMD for their North American isolate. Hypocrea pilulifera J. Webster & Rifai, Trans. Brit. Mycol. Soc. 51: 511 (1968). Fig. 49 Fig.
49 Teleomorph of Hypocrea pilulifera. a–d. Fresh stromata (a, d. immature, b. partly immature). e–j. Dry stromata (e. immature, with stipe-like base). k. Rehydrated stroma. l. Stroma in 3% KOH after rehydration. m. EMD 1214063 Stroma surface in face view. n. Perithecium in section. o. Cortical and subcortical tissue in section. p. Subperithecial tissue
in section. q. Stroma base in section. r–t. Asci with ascospores (t. in cotton blue/lactic acid). u, v. Ascospores (u. vital, multiguttulate; v. cells distinctly dimorphic; viable and dead). a, b, d, f, h, k–q, v. WU 29408. c, e, g, j, r, t, u. WU 29409. i, s. Holotype K 64379. Scale bars a, b, k, l = 1 mm. c, h = 0.6 mm. d, i = 0.3 mm. e–g, j = 0.4 mm. m, r–v = 10 μm. n, q = 40 μm. o, p = 20 μm Anamorph: Trichoderma piluliferum J. Webster & Rifai, Mycol. Pap. 116: 16 (1969). Fig. 50 Fig. 50 Cultures and anamorph of Hypocrea pilulifera (CBS 120927). a–c. Cultures (a. CMD, 25 days. b. PDA, 28 days. c. SNA, 25 days). d. Conidiation pustule on SNA (18 days). e, f. Conidiophores with elongations on pustule selleckchem margins on growth plate (f. young, showing right-angled branching; 18 days). g–k. Conidiophores (18 days; g. showing sterile elongations). l. Phialides (18 days). m, n. Chlamydospores (21 days). o, p. Conidia (25°C, 45 days). a–p. All at 15°C except o, p. d–p. All on SNA. Scale bars a–c = 15 mm. d = 0.5 mm. e, g, h = 30 μm. f = 70 μm. i, k, n = 15 μm. j, l, m = 10 μm. o,
p = 5 μm Stromata when fresh 1–5 mm diam, 1–1.5 mm thick, pulvinate, broadly attached, margin free, surface smooth, ostiolar dots distinct, first watery, yellowish to olive-greenish, later ochre to brown. Stroma colour first white, turning light yellow, nearly citrine, 2–3A2–4, cream or argillaceous when mature, mostly 4AB4. Stromata when dry (0.7–)1.5–3.4(–4.0) × (0.6–)1.2–2.6(–3.5) mm, (0.3–)0.5–1.1(–1.5) mm thick (n = 44), solitary, scattered or aggregated in small numbers (2–3), pulvinate or discoid, broadly attached; outline circular or oblong; rarely with radiating white basal mycelium. Edges free, sides rounded or straight vertical, smooth, sometimes present as a white broad stipe-like base with the apical fertile part laterally projecting over it.