Table 1 This table shows demographic and strength data of the study participants. Participant Demographics and Strength Measures Age 22.5 ± 2.2 Height (m) 1.77 ± .06 Weight (kg) 84.4 ± 13.6 Squat 1RM (kg) 125.2 ± 33.9 Leg Press 1RM (kg) 254.9 ± 80.2 Leg Extension 1RM (kg) 112.0 ± 26.9 Values are expressed as mean ± standard deviation. Familiarization
Participants in this study AZD1208 were asked to arrive at the Human Performance Research Laboratory (HPRL) at West Texas A&M University having fasted overnight. Participants underwent a fasting venous blood draw collected from the antecubital fossa, to determine pre-supplementation plasma cortisol and testosterone levels. Additionally, participants were required to perform 1 repetition maximum (RM) lifts in the Smith machine squat (SQ), leg press (LP), and leg extension (LE) exercises after performing a standardized warm up of walking briskly on a treadmill for five minutes. 1RM testing followed the National Strength and Conditioning Associations guidelines. Participants also performed a Serial Subtraction Test and a Profile of Mood States questionnaire to familiarize themselves with these instruments. Supplementation
Protocol Following familiarization, participants were randomly assigned to consume PS or PL for 14 days each. Following 14 days of supplementation with their first assigned supplement, participants reported to the HPRL for their first testing session. Upon completion of the first testing Selleck Tanespimycin session, participants were given a 14 day supply of either PS or PL, depending on which supplement they took for the previous 14 days. No washout period was followed after the first supplementation period. After completing the 14 day supplementation period with the other supplement, participants reported to the HPRL for their second and final testing session. Cognitive Function and
Mood Measurement In order to analyze cognitive function, a Serial Subtraction Test (SST) was used. This consisted of a two minute timed test in which the participants subtracted the number 7 from a random 17-DMAG (Alvespimycin) HCl 4 digit number in order to measure how quickly and accurately they can compute a simple mathematical problem. The average time per correct calculation, number of correct calculations, and number of mistakes were recorded. If an incorrect calculation was made, subsequent calculations were deemed correct based on the new starting number [7]. Analysis of mood was performed by administering the Profile of Mood States (POMS) questionnaire. The POMS measurement is used to measure transient mood states and measures six factors including: tension, depression, anger, fatigue, vigor, and confusion. The POMS has been used extensively in the past to examine changes in mood states as a result exercise [8]. Testing Sessions On both the first and second testing sessions, participants reported to the HPRL in a fasted state.