selleck products electrodes with higher sheet resistances and electrodes subject to higher current densities fail more quickly. The reason for electrode failure is attributed to the instability of silver nanowires

at elevated temperatures caused by Joule heating. Design factors such as passivation, electrode sheet resistance, and nanowire diameter need to be considered before silver nanowire electrodes will be useful as an ITO replacement in organic solar cells. Endnotes aThe current density in the nanowires was estimated by dividing the total current flowing across the electrode by the total cross-sectional area of all nanowires contacting the copper strip at one end of the sample Selleck 4SC-202 and multiplying by two since we assumed only half of the nanowires were involved in conduction. Acknowledgements This work was supported by the Natural Science and Engineering Research Council (NSERC) of Canada. References 1. Hecht DS, Hu L, Irvin G: Emerging transparent electrodes based on thin films of carbon nanotubes, graphene, and metallic nanostructures. Adv Mater 2011, 23:1482–1513.CrossRef learn more 2. Kumar A, Zhou C: The race to replace tin-doped indium oxide:

which material will win? ACS Nano 2010, 4:11–14.CrossRef 3. Hu L, Kim HS, Lee JY, Peumans P, Cui Y: Scalable coating and properties of transparent, flexible, silver nanowire electrodes. ACS Nano 2010, 4:2955–2963.CrossRef 4. Hardin BE, Gaynor W, Ding I, Rim SB, Peumans P, McGehee MD: Laminating solution-processed silver nanowire mesh electrodes onto solid-state dye-sensitized solar cells. Org Electron 2011, 12:875–879.CrossRef 5. Yu Z, Li L, Zhang Q, Hu W, Pei Q: Silver nanowire-polymer composite Acyl CoA dehydrogenase electrodes for efficient polymer solar cells. Adv Mater 2011, 23:4453–4457.CrossRef 6. Elechiguerra JL, Larios-Lopez L, Liu C, Garcia-Gutierrez D, Camacho-Bragado A, Yacaman

MJ: Corrosion at the nanoscale: the case of silver nanowires and nanoparticles. Chem Mater 2005, 17:6042–6052.CrossRef 7. Green MA, Emery K, Hishikawa Y, Warta W, Dunlop ED: Solar cell efficiency tables (version 39). Prog Photovolt Res Appl 2012, 20:12–20.CrossRef 8. Dan B, Irvin GC, Pasquali M: Continuous and scalable fabrication of transparent conducting carbon nanotube films. ACS Nano 2009, 3:835–843.CrossRef 9. Liu CH, Yu X: Silver nanowire-based transparent, flexible, and conductive thin film. Nanoscale Res Lett 2011, 6:1–8. 10. Zeng XY, Zhang QK, Yu RM, Lu CZ: A new transparent conductor: silver nanowire film buried at the surface of a transparent polymer. Adv Mater 2010, 22:4484–4488.CrossRef 11. Krantz J, Richter M, Spallek S, Spiecker E, Brabec CJ: Solution-processed metallic nanowire electrodes as indium tin oxide replacement for thin-film solar cells. Adv Funct Mater 2011, 21:4784–4787.CrossRef 12. Patil HR, Huntington HB: Electromigration and associated void formation in silver. J Phys Chem Solids 1970, 31:463–474.

Clin Exp Metastasis 2005, 22: 503–511.CrossRefPubMed 20. Hata K, Dhar DK, Watanabe Thiazovivin purchase Y, Nakai H, Hoshiai H: Expression of metastin and a G-protein-coupled receptor (AXOR12) in epithelial ovarian cancer. Eur J Cancer 2007, 43: 1452–1459.CrossRefPubMed 21. Schmid K, Wang X, Haitel A, Sieghart W, Peck-Radosavljevic M, Bodingbauer M, Rasoul-Rockenschaub S, Wrba F: KiSS-1 overexpression as an independent prognostic marker in hepatocellular carcinoma: an immunohistochemical study. Virchows Arch 2007, 450: 143–149.CrossRefPubMed 22. Dhillo WS, Murphy KG, Bloom SR: The neuroendocrine physiology of kisspeptin in the human. Rev Endocr Metab Disord

2007, 8: 41–46.CrossRefPubMed 23. Mead EJ, Maguire JJ, Kuc RE, Davenport AP: Kisspeptins: a multifunctional peptide system with a role in reproduction, cancer and the cardiovascular system. Br J Pharmacol 2007, 151: 1143–1153.CrossRefPubMed 24. Masui T, Doi R, Mori T, Toyoda E, Koizumi M, Kami K, Ito D, Peiper SC, Broach JR, Oishi S, Niida A, Fujii N, Imamura M: Metastin and its variant forms suppress migration Pinometostat mw of pancreatic cancer cells. Biochem Biophys Res Commun 2004, 315: 85–92.CrossRefPubMed 25. Katagiri F, Tomita K, Oishi S, Takeyama M, Fujii N: Establishment and clinical application of enzyme immunoassays for determination of luteinizing hormone releasing hormone and metastin.

J Pept Sci 2007, 13: 422–429.CrossRefPubMed 26. International Union Against Cancer (UICC): TNM Classification of Malignant Tumours. 6th edition. New York: Wiley-Liss; 2002. 27. Kitagawa T, Shimozono T, Aikawa T, Yoshida T, Nishimura H: Preparation and characterization of hetero-bifunctional cross-linking reagents for protein modifications. Chem Pharm

Bull 1981, 29: 1130–1135. 28. Harms JF, Welch DR, Miele ME: KISS1 metastasis suppression and emergent pathways. Clin Exp Metastasis 2003, 20: 11–18.CrossRefPubMed 29. Stafford LJ, Xia C, Ma W, Cai Y, Liu M: Identification and characterization of mouse metastasis-suppressor KiSS1 and its G-protein-coupled receptor. Cancer Res 2002, 62: 5399–5404.PubMed 30. Yan C, Wang H, Boyd DD: KiSS-1 represses 92-kDa type IV collagenase expression by down-regulating NF-kappa B binding to the promoter as a consequence of Ikappa Balpha -induced block of p65/p50 nuclear translocation. J Biol Thymidine kinase Chem 2001, 276: 1164–1172.CrossRefPubMed 31. Bilban M, Ghaffari-Tabrizi N, Hintermann E, Bauer S, Molzer S, Zoratti C, Malli R, Sharabi A, Hiden U, Graier W, Knofler M, Andreae F, Wagner O, Quaranta V, Desoye G: Kisspeptin-10, a KiSS-1/metastin-derived decapeptide, is a Stem Cells antagonist physiological invasion inhibitor of primary human trophoblasts. J Cell Sci 2004, 117: 1319–1328.CrossRefPubMed 32. Koshiba T, Hosotani R, Wada M, Miyamoto Y, Fujimoto K, Lee JU, Doi R, Arii S, Imamura M: Involvement of matrix metalloproteinase-2 activity in invasion and metastasis of pancreatic carcinoma. Cancer 1998, 82: 642–650.CrossRefPubMed 33.

All authors read and approved the final manuscript.”
“Background Aerobic anoxygenic photoheterotrophic bacteria use light as additional energy source for mixotrophic growth and play a significant

role in the microbial ecology of marine environments [1, SIS3 concentration 2]. Members of this physiological group belonging to the Alphaproteobacteria have been intensively studied (for review see e.g.[3, 4]), but so far little is known on the phenotypic diversity of representatives belonging to the Gammaproteobacteria. The existence of aerobic anoxygenic photoheterotrophic gammaproteobacteria in marine environments was first postulated in a study by Béjà et al. [5], who could identify photosynthesis genes in partial genome sequences of gammaproteobacteria retrieved from seawater off the coast of California (USA). A few years later the two marine isolates HTCC2080 and KT71T were independently identified as aerobic anoxygenic photoheterotrophic gammaproteobacteria by proteomic analyses [6] and genome sequencing [7], respectively. Strain KT71T was subsequently characterized in detail and Selleckchem Bortezomib described as Congregibacter litoralis (C. litoralis) by Spring Epigenetics inhibitor et al. [8], thereby representing the first photoheterotrophic bacterium of this group with a validly

published name. Phylogenetically, C. litoralis is affiliated to a large coherent cluster of 16S rRNA gene sequences, which were mainly retrieved by cultivation-independent Thymidine kinase methods from marine habitats around the world. This sequence cluster was recognized as a distinct lineage within the class Gammaproteobacteria and designated as OM60 [9, 10] or NOR5 clade [11]. Metabolic active bacteria representing

this clade could be detected in numerous environmental samples by using fluorescence in situ hybridization experiments [12, 13]. Based on these findings it is assumed that the OM60/NOR5 clade of Gammaproteobacteria is of significant ecological importance due to its widespread occurrence in the euphotic zone of saline ecosystems and high abundance especially in coastal waters [6, 13, 14]. A phylogenetic lineage closely related to the OM60/NOR5 cluster was originally defined by a 16S rRNA gene sequence retrieved from deep sea sediment and designated BD1-7 [13]. In recent years reports about the isolation of additional strains belonging to the OM60/NOR5 group have accumulated. Some of these strains were described as mixotrophs containing photosynthetic pigments [6, 15] or proteorhodopsin (PR) [16]. In contrast, no photosynthetic pigments were reported in members of the genus Haliea[17–19] or Halioglobus[20].

​org.​uk/​, John van Wyhe, director). J.P. acknowledges the financial click here support by grants BFU2006-01951/BMC from the Spanish Ministry of Science and Innovation and FP7-KBBE-2007-212894 (TARPOL project, European Union). The support of the Institut Pasteur-Fondazione Cenci Bolognetti (Universita di Roma, La Sapienza) and the generous hospitality of Professor Ernesto VX-689 di Mauro (Universita di Roma, La Sapienza) to A.L. are gratefully acknowledged. Open Access This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source

are credited. References Aulie R (1970) Darwin and spontaneous generation. J Amer Sci Affil 22:31–33 Bastian HC (1907) The evolution of life. P. Dutton and Co, New York Bronn HG (1860) [Review of] Ch. Darwin: on the origin of species by means of natural selection, or the preservation of favoured races in the struggle for life (London 1859). Neues Jahrbuch Selleckchem AZD0530 für Mineralogie, Geognosie, Geologie und Petrefaktenkunde:112–116 [Translated in David Hull, 1973. Darwin and His Critics: The Reception of Darwin’s Theory of Evolution by the Scientific Community. University of Chicago Press, Chicago pp. 120–124] Calvin M

(1969) Chemical evolution: Molecular evolution towards the origin of living systems on the Earth and elsewhere. Oxford University Press, New York Crowe MJ (1986) The extraterrestrial life debate 1750–1900: The idea of a plurality of worlds from Kant to Lowell.

Cambridge University Press, Cambridge Dahm R (2005) Friedrich Miescher and the discovery of DNA. Dev Biol 278:274–288PubMedCrossRef Darwin Ch (1863) The doctrine of heterogeny and modification of species. Athenæum no. 1852, 25 April 1863:554–555. [Reprinted in: van Wyhe J 2009:334–337] Darwin Ch (1868) The variation of animals and plants under domestication, 2 vols. Murray, London Darwin F (ed) (1887) The life and letters of Charles Darwin, including an autobiographical chapter, 3 vols. (-)-p-Bromotetramisole Oxalate John Murray, London De Beer G (1959) Some unpublished letters of Charles Darwin. Notes Rec R Soc Lond 14:12–66CrossRef de Beer G (ed) (1960) Darwin’s notebooks on transmutation of species. Part IV, Fourth notebook [E] (October 1838–10 July 1839). Bull Brit Mus (Nat Hist) Hist Ser 2: 151–183 de Beer G, Rowlands MJ, Skramovsky BM (eds) (1967) Darwin’s notebooks on transmutation of species. Part VI. Pages excised by Darwin. Bull Brit Mus (Nat Hist) Hist Ser 3:129–176 Farley J (1977) The spontaneous generation controversy: from Descartes to Oparin. Johns Hopkins University Press, Baltimore Haeckel E (1862) Die Radiolarien (Rhizopoda Radiaria). Eine Monographie. Druck und Verlag Von Georg Reimer, Berlin Lazcano A (2002) Foreword to Lynn Margulis and Michael Dolan’s early life.

During

infection, the ability of macroautophagy to remove large cytoplasmic structures with selectivity enables this pathway to be used to clear intracellular bacteria, parasites, and viruses (i.e., xenophagy) [1, 8, 9]. Several medically important human pathogens are degraded in vitro by xenophagy, including bacteria (e.g., group A streptococcus, Mycobacterium tuberculosis, Shigella flexneri, selleck inhibitor Salmonella enterica, Listeria monocytogenes, and LEE011 Francisella tularensis), viruses such as herpes simplex virus type 1 (HSV-1) and chikungunya virus, and parasites such as Toxoplasma gondii[9]. We therefore wondered whether induction of autophagy could affect the growth of E. coli in infected HMrSV5 cells. We found that stimulation of autophagy by LPS in infected HMrSV5 cells could lead to degradation of E. coli within autophagosomes. Furthermore, we observed that 3-MA or Wm blockade of autophagy markedly attenuated the co-localization of E. coli with autophagosomes, leading to a defect in bactericidal activity. To more specifically determine whether autophagy affect the elimination of E.coli, Beclin-1 siRNA was employed to inhibit autophagy. As expected, fewer E.coli were targeted to the autophagosomes, and consequently more remaining

E.coli were observed in cells deficient in Beclin-1. Taken together, these data demonstrated that the effect of LPS on bactericidal check details activity was dependent on the induction of autophagy. LPS is the ligand for TLR4, and it also exerts multiple cellular

effects by inducing signaling through TLR4 [10]. The activation of TLR4 by LPS in peritoneal mesothelial cells might result in a massive influx of leukocytes in the peritoneal cavity, leading to the development of peritoneal dysfunction or peritoneal fibrosis [28]. It was demonstrated that TLR4 served as a previously unrecognized environmental sensor for autophagy [10]. Therefore we further investigated whether TLR4 played roles in LPS-induced autophagy in HMrSV5 cells. Our results showed that the LPS treatment increased the expression of TLR4 protein significantly in a dose-dependent and time-dependent way. of Moreover, the increased expression of TLR4 protein occurred earlier than the increase of LC3-II protein. Pretreated with PMB, a TLR4 inhibitor, displayed defective autophagy activation as indicated by the significantly decreased expression of both Beclin-1 and LC3-II protein as well as the decreased GFP–LC3 aggregation in cells. Consistent with the pharmacological inhibition of TLR4, knockdown of TLR4 with TLR4 siRNA also led to reduction of autophagy-associated proteins. Importantly, LPS-induced bactericidal activity in HMrSV5 cells was significantly decreased after knockdown of TLR4. To sum up, these results demonstrated that upregulation of autophagic response by LPS was dependent on TLR4 signaling in HMrSV5 cells.

First is that the AZO film was deposited on the amorphous quartz substrate, which results in a polycrystalline AZO film as discussed below. Figure 1h is a typical AFM surface image of an AZO film. AFM results indicate that the root-mean-square surface roughness and the average surface particle size are 10.2 and 140 nm, respectively. The second reason, therefore, is that the polycrystalline AZO film deposited by RF sputtering has large surface roughness and surface particle size. In a MK-2206 hybrid solar cell, ZnO NRs play the roles to extract carriers

from the absorber and provide a fast and direct path for these carriers. The efficiency of a solar cell strongly relies on the crystallinity, density, diameter, and A-1210477 concentration length

of ZnO NR [9, 15]. Conradt et al. [15] have reported Captisol datasheet that short NRs in the range of 100 to 500 nm are of particular interest for hybrid solar cells. A smaller NR diameter will enhance the spacing between NRs and increase the solar absorber amount and the efficiency of a solar cell [9]. NR in sample S3 has a suitable length about 500 nm and a small diameter about 26 nm. Accordingly, we suggest that sample S3 is interesting for application in hybrid solar cells. Most NRs in sample S4 are well aligned, as shown in Figure 1d. However, the phenomenon of two or three NRs self-attracting can be seen obviously in the inset of Figure 1d. Han et al. [22] and Wang et al. [23] had reported self-attraction among aligned ZnO NRs under an electron beam, while Liu et al. [24] have observed the self-attraction of ZnO

NWs after the second-time growth. In our samples, NRs with a relatively small diameter are slightly oblique and easily bent, which results in NR self-attraction, given that the NRs are long enough. According to the experimental observation, we propose two possible NR self-attraction models, as presented in Figure 2. The insets in Figure 2 are top-view images of sample S4, and the arrows in the insets denote the examples of the self-attraction models. In the first case, in Figure 2a, NRs randomly grow and are slightly tilted, so the tips of two NRs may just touch each other when the NRs are long enough. In the second case, a NR body may slightly bend due to the oblique growth, which causes the side Oxalosuccinic acid surfaces to be either positively or negatively charged because of the piezoelectric properties of ZnO NRs [13, 24]. As a result, as indicated in Figure 2b, when two bending NRs cross, the opposite charges will lead to the attraction at the crossed position due to the large electrostatic force. Figure 2 Schematic diagrams of two possible NR self-attraction models. (a) The tips of two NRs touch each other, (b) two NRs touch each other at the crossed position. Insets are top-view images of sample S4. Figure 3 presents XRD patterns of an AZO film along with the samples.

Before the anodization process, the silicon 17DMAG substrate was immersed in HF solution for 2 min to remove the native oxide

layer. Since the PSi fabrication process is self-stopping, it is possible to obtain adjacent layers with different porosities by changing the current density during the electrochemical etching [4]. A current density of 200 mA/cm2 for 1.2 s was applied to obtain low refractive index layers (n L = 1.542; d L = 125 nm) while a current density of 100 mA/cm2 was applied for 1.4 s for high refractive index layers (n H = 1.784; d H = 108 nm). After the electrochemical process, the pore dimension was increased to favour the infiltration of biological matter by rinsing the fresh-made PSi microcavities in a KOH ethanol solution (1.5 mM) for 15 min [5]. The structures were then thermally oxidized against uncontrolled environmental

aging and corrosion in alkaline solutions. The thermal oxidation has been performed in pure O2 by a two-step process: pre-oxidation at 400°C for HDAC inhibitor 30 min followed by oxidation at 900°C for 15 min. Silane surface modifications Eight oxidized PSi microcavities (PSi-Ma-h) were immersed in piranha solution (H2O2:H2SO4 1:4) at RT for 30 min to generate Si-OH groups on the PSi surface. After that, the samples were extensively washed in Milli-Q® water flow (Millipore, Billerica, MA, USA) and dried with nitrogen gas. Structures were then CB-5083 molecular weight silanized by immersion in different 5% aminosilane solutions, (3-aminopropyl)triethoxysilane

(APTES) or (3-aminopropyl)-dimethyl-ethoxysilane (APDMES), in dry toluene for 30 min at RT. Samples PSi-Ma,c,e,g were silanized by APTES and samples PSi-Mb,d,f,h by APDMES. The reaction conditions were optimized on a crystalline silicon-varying solvent for silane dissolution and incubation time [12]. The PSi-silanized samples were rinsed three times in the solvent used for the process so as to remove the ungrafted Farnesyltransferase silanes. The last step of silanization is curing at 100°C for 10 min. Oligonucleotide synthesis Chemicals and solvents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Reagents and phosphoramidites for DNA synthesis were purchased from Glen Research (Sterling, VA, USA). Solid-phase ON syntheses were performed on a PerSeptive Biosystem Expedite 8909 DNA automated synthesizer (Framingham, MA, USA). The 19-mer mixed-sequence oligonucleotide 5′-GATTGATGTGGTTGATTTT-3′ was assembled on two different aminosilane-modified microcavities, following phosphoramidite chemistry by 19 growing cycles [13]. PSi structures, PSi-Mg,h-NH2 (Mg = APTES, Mh = APDMES), were introduced in a suitable column reactor to be used in the automated synthesizer; the syntheses were performed according to the scheme reported in Figure 1. In all cases, the first reaction step involved the attachment of the 3′-ending nucleobase to the amino group of PSi-bound APTES or APDMES.

In most cases this procedure yielded ca. 10 μg of extracted total RNA as determined by photometric analysis at 260 nm. Despite the applied on-column

DNase treatment small quantities of genomic DNA could still be detected in the purified RNA samples by PCR amplification. Hence, an additional DNase treatment in solution was applied to obtain DNA-free RNA. Selleck LY411575 Reverse transcriptase-PCR (RT-PCR) of mRNA was performed with the OneStep RT-PCR kit of Qiagen following the instructions given by the manufacturer and using 0.5 μg of total RNA. Gene-specific selleckchem primers are listed in Table 1 and the following thermal cycler conditions were used for amplification: reverse transcription at 50°C for 30 min, an initial step at 95°C for 15 min and then 30 cycles at 94°C for 30 s, 58°C for 1 min and 72°C for 1 min. At the end a postelongation at 72°C for 5 min was carried out. RT-PCR products were visualized using the FlashGel electrophoresis system with DNA Cassettes (2.2% agarose) from Lonza (Verviers, EPZ-6438 concentration Belgium) and a Kodak EDAS 290 imaging system. Normalization of mRNA levels was performed using specific rpoZ primers (Table 1), which amplify the omega subunit of the RNA polymerase, a housekeeping gene that seems to be expressed constitutively

in a Rhodobacter species [32]. Table 1 Oligonucleotides used for the amplification of gene fragments from C. litoralis DSM 17192 T with PCR or semiquantitative RT-PCR Primer Sequence (5′-3′) Ta(°C) Protein encoded by the target gene Product size (bp) KT71 rpoZ-F CAT CAC TTC GGC GAG TTC TT 58 RNA many polymerase omega subunit 223 KT71 rpoZ-R AGA AGA TTG CCT TGA GTC CG KT71 cycB1-F GAC AGT CGG TTT GAT TGC AG 58 Cytochrome c 5 204 KT71 cycB1-R CAT GCG GTG TTG

TAA GTT GC KT71 pufC-F AAG CAG ACC GAG TGG ACC TA 58 Photosynthetic reaction centre cytochrome c subunit 373 KT71 pufC-R GTG CCT TCT CAG ACT CCG TC KT71 ctaD-F ATA TCC ACT TTG GCA CCA GC 58 Caa 3-type cytochrome c oxidase subunit 1 409 KT71 ctaD-R GTG AAG AGC ACA AGG AAG CC KT71 ccoN1-F CTT ATC ACC GTC GTC TGG GT 58 Cbb 3-type cytochrome oxidase CcoN subunit 392 KT71 ccoN-R GTG TAG TGC AGG TGG TGT GG Ta indicates the annealing temperature used in the PCR reaction. Acknowledgements TR was supported by the DFG Transregio-SFB 51 Roseobacter. References 1. Jiao N, Zhang Y, Zeng Y, Hong N, Liu R, Chen F, Wang P: Distinct distribution pattern of abundance and diversity of aerobic anoxygenic phototrophic bacteria in the global ocean. Environ Microbiol 2007, 9:3091–3099.PubMedCrossRef 2. Lami R, Cottrell MT, Ras J, Ulloa O, Obernosterer I, Claustre H, Kirchman DL, Lebaron P: High abundances of aerobic anoxygenic photosynthetic bacteria in the South Pacific Ocean. Applied Environ Microbiol 2007, 73:4198–4205.CrossRef 3.

In: Lehman SM, Fleagle JG (eds) Primate biogeography. PF299804 price Springer, New York, pp 331–372 Haywood AM, Dowsett HJ, Valdes PJ, Lunt DJ, Francis JE, Sellwood BW (2009) Introduction. Pliocene climate, processes and problems. Philos Trans R Soc A 367:3–17 Heaney LR (1991) A synopsis of climatic and vegetational change in Southeast Asia. Climatic Change 19:53–61 Heaney LR click here (2004) Conservation biogeography in oceanic archipelagoes. In: Lomolino MV, Heaney LR (eds) Frontiers of biogeography. Sinauer, Sunderland, MA, pp 345–360 Hill C, Soares P,

Mormina M, Macaulay V, Meehan W, Blackburn J, Clarke D, Raja JM, Ismail P, Bulbeck D, Oppenheimer S, Richards M (2006) Phylogeography and ethnogenesis of aboriginal southeast Asians. Mol Biol Evol 23:2480–2491PubMed Hirsch P (ed) (1997) Seeing forests for trees: environment and environmentalism in Thailand. Silkworm Books, Chiang Mai and University of Washington Press, Seattle,

p 277 Hirsch P, Warren C (eds) (1998) The politics of environment in Southeast Asia: resources and resistance. Routledge, New York Hofreiter M, Stewart J (2009) Ecological change, range fluctuations and population dynamics during the Pleistocene. Curr Biol 19:R584–R594PubMed Hoglund J (2009) Evolutionary conservation genetics. Oxford University Press, Oxford Holloway JD (2003) An addiction to Southeast Asian biogeography. Introduction to a collection of papers originated in the conference, Biogeography of Southeast Asia—organisms and orogenesis, held in The Netherlands on 4–9 June 2000. J Biogeogr 30:161–163 SB203580 in vivo Horton BP, Gibbard PL, Milne GM, Morley RJ, Purintavaragul C, Stargardt JM (2005) Holocene sea levels and palaeoenvironments, Malay-Thai

Peninsula, Southeast Asia. Holocene 15:1199–1213 Hubbell SP (2001) The unified neutral theory of biodiversity and biogeography. Princeton University Press, Princeton Hughes JB, Round PD, Woodruff DS (2003) The Indochinese-Sundaic faunal transition at the Isthmus of Kra: an analysis of resident forest bird species Reverse transcriptase distributions. J Biogeogr 30:569–580 Hutchison CS (1989) Geological evolution of south-east Asia. Clarendon, Oxford Kawecki TJ (2008) Adaptations to marginal habitats. Annu Rev Ecol Evol Syst 39:321–342 Kershaw AP, Penny D, van der Kaars S, Anshari G, Thamotherampilai A (2001) Vegetation and climate in lowland southeast Asia at the last glacial maximum. In: Metcalfe I, Smith JMB, Morwood M, Davidson I (eds) Faunal and floral migrations and evolution in SE Asia-Australasia. Balkema, Lisse, pp 227–236 Kershaw AP, van der Kaars S, Flenley JR (2007) The Quaternary history of far eastern rainforests. In: Bush MB, Flenley JR (eds) Tropical rainforest responses to climate change. Springer, Berlin, pp 77–115 Kottelat M (2002) Aquatic systems: neglected biodiversity. In: Wikramanayake E et al (eds) Terrestrial ecoregions of the Indo-Pacific.

HH participated in the analysis

of TEM results. WZ deposited the Al-doped ZnO films. YQ participated in the test of the samples. FL designed the study and drafted the manuscript. All authors read and approved the final manuscript.”
“Background Rapid advances on the many fronts in the field of GaN-based technology, including in the growth of materials, have promoted the commercialization of green and blue light-emitting diodes (LEDs) and laser diodes [1]. Sapphire has been the most extensively used substrate for GaN growth owing to its relatively low cost, chemical compatibility, and stability at high temperatures. Despite considerable progress in the field of GaN-based technology, major obstacles to the realization of the full potential of these GaN-based materials are Palbociclib mw present. One of the greatest problems is the lack of a suitable substrate material on which lattice-matched GaN films can be grown. RG-7388 GaN heteroepitaxial films that are grown on sapphire substrate using various growth techniques

have been studied widely [1–5]. The preparation of the surface of the substrate is a critical consideration in maximizing the quality of epitaxial films. To increase the internal quantum efficiency and light extraction efficiency of GaN-based LEDs, they are fabricated on a patterned sapphire substrate (PSS) [3–6]. Air gaps between GaN and the sapphire substrate can be formed by geometrically patterning the substrate to release the internal

stress that find more is associated with the lattice mismatch that exists at the air gap, reducing the dislocation density and improving the quality of the film. Total internal reflection easily occurs in a traditional LED, so the reflection of light therein is difficult, and some light is even absorbed by the film in the LED structure. A patterned substrate can form a light-scattering area by geometry on the substrate and increase the probability of the light leaving the LEDs inside to improve the light power [7, 8]. Patterned substrates can be formed by two categories of methods – dry etching and wet etching [9]. Dry etching is a method in which a gaseous chemical etching agent is used to perform non-isotropic etching, but it is likely to destroy the surface and form defects. DNA ligase Wet etching uses a chemical solution to etch the surface of a semiconductor isotropically; the etching rate is a function of the temperature and concentration of the solution. Such methods typically have a very high selectivity and etching rate. The etching process comprises two steps, which are [10] (1) the diffusion of the chemical etching solution to the surface of the material that is to be etched and (2) the reaction of the chemical etching solution with the materials. Wet etching is divided into mask-associated etching and mask-free etching [10–12]. Mask-associated etching utilizes a circular array of SiO2 on the surface of a sapphire substrate as an etching barrier layer.