, 2005). SecA was identified in infected duck livers of R. anatipestifer
by SCOTS (Zhou et al., 2009). The tad (tight adherence) locus is necessary for adherence and the biogenesis of the Flp pilus and includes the tadD and tadG genes (Wang & Chen, 2005). A tadD mutant of P. multocida was attenuated in mice in STM (Fuller et al., 2000). The inactivation of tadG leads to excessive secretion Seliciclib purchase of matrix materials (Wang & Chen, 2005). The putative glp genes, including glpA, glpB, glpC, glpK, and glpT, encode subunits of the anaerobically expressed glycerol-3-phosphate dehydrogenase. The genes glpA, glpB, and glpC were significantly downregulated in chickens as detected by DNA microassay (Boyce et al., 2002). The glpT and glpK genes were identified in this study and in S. suis by SCOTS, respectively (Li et al., 2009). Until recently, the identification of differential gene expression in bacteria within infected host cells or tissues has been limited by the low number of bacteria in these systems and the instability of bacterial mRNA. There are also difficulties involved in separating bacterial mRNA from ribosomal RNA (rRNA) and host RNA. In summary, our study confirmed that
the SCOTS approach is an economical, direct approach by which to identify genes expressed by a given organism in response to specific environmental conditions that is widely applicable to virtually any prokaryote find more and to other organisms as well, for example, the rabbit liver. Further SCOTS experiments to identify P. multocida genes expressed differentially in different tissues, as well as in earlier or later stages of infection, will help to elucidate the mechanisms of pathogenesis for this economically significant bacterium. Thirty-one P. multocida genes were identified that were up-regulated, and this provides a valuable starting point for determining their function and whether they have a role in virulence. We will focus on the major role of cell surface
biosynthesis and the presence of a general sensor-effector system in bacteria, which is important in their potential role as vaccine candidates. Thalidomide “
“Although carbendazim (MBC) and other benzimidazole fungicides have effectively controlled bakanae disease of rice (which is caused by Fusarium fujikuroi, F. proliferatum, and F. verticillioides) in the past, MBC resistance has become common. Previous research has shown that MBC resistance results from a mutation in the β1-tubulin (β1tub) gene in F. verticillioides. However, MBC resistance in F. fujikuroi, a predominant species in China, does not result from a mutation in the β1tub. The molecular mechanism of F. fujikuroi resistance against benzimidazole fungicides is poorly understood. In this study, we determined that although β1tub and β2-tubulin (β2tub) in F. fujikuroi have high homology with β1tub and β2tub in F. verticillioides, MBC resistance in F.