we discovered a dose dependent decrease in area of the nuclei of both treated HGPS and treated handle cells, the area of mock treated nuclei was greater than both doses of RAD001 selective Aurora Kinase inhibitors treated nuclei, but the nuclei that received the smaller dose of RAD001 had greater area than the nuclei that received the larger dose. This result indicates the improvement in nuclear form is just a continuous process, the region reduction is mostly due to non specific ramifications of the drug treatment, and incrementtal improvement all through treatment could be captured and quantified by this curvature outline imaging analysis. One of the hallmarks of HGPS could be the abnormal nuclear form known as blebbing. This has been the principle morphological feature pinpointing an HGPS cell line and has been used to ascertain the potency of treatments for HGPS. The standard method of measuring blebbing is just a information, blind count of the proportion of blebbed nuclei. Nevertheless, this process does not have any common requirements and is extremely time consuming. Selecting the nuclei into two groups, blebbed and typical, also obscures the truth that blebbing is not an either/or phenomenon, but varies continuously. The subjectivity and variability resonance of the limit for blebbed nuclei causes it to be impossible to compare values obtained by different counters. . The necessity for a fair, quantitative method of measuring the degree of blebbing in a cell sample is clear. In a effort towards solving this matter, we present an automated image analysis method while the primary measure of blebbing using curve. We used a custom written system to assess a curve Vortioxetine curve and get the boundaries of immuno stained nuclei for every nucleus among other measures of form. . We found that several measures of the form distinguish between HGPS and normal get a grip on cell lines. We focused on probably the most intuitive measure, the mean negative curvature, which will be the average of all the curvatures on the boundary of a nucleus. MNC provides a constant measure of blebbing that can be found in quantitative and mathematical methods. We assessed various seeding densities and exposure times to show that MNC is also a frequent measure that doesn’t differ somewhat between trials. The cluster analysis also suggests that intensity doesn’t influence the measured MNC. Therefore MNC values could be compared between samples and tests, unlike values obtained in the old-fashioned blebbing count technique. One caveat is that MNC is affected by smoothing and pixel dimension, thus care must be taken when comparing results from different laboratories. Of another measures that strongly correlate with MNC, based on our clustering analysis, solidity shouldn’t be substantially affected by pixel size or smoothing and thus may be a viable alternative.