The latter case assumes that msRNA-428 may be produced in the course of degradation; however, its discrete size and cellular abundance argue for controlled processing and putative independent functioning. A complete data list, including ID, representative clone sequence, location in the 5′- and 3′-strand duplex of each msRNA hairpin loop, clone count, extended sequence and hairpin formation are presented Talazoparib cost in Table S1, which can be viewed online. Deep sequencing (next-generation sequencing) has given new opportunities to identify and quantify miRNAs (or sRNAs). With this technique, we analysed small-size, noncoding RNAs in an oral pathogen. By sequencing cDNA

libraries prepared from size-fractionated S. mutans RNA, we identified more than 900 possible msRNAs. Despite intensive studies of miRNAs in eukaryotic cells and viruses, the functions of sRNAs in bacteria remain largely uncharacterized except in E. coli. The c. 22 nt miRNAs employ well-established mechanisms to repress the mRNAs by short

seed pairing (animal) or intensive pairing (plant) within the 3′ untranslated region (Bartel, 2009). In bacteria, sRNAs are often bound to the RNA chaperone protein Hfq, which stabilizes their folding (Gottesman, 2004). Bacterial sRNAs form complementary duplexes with their target RNAs most frequently at the 5′ end of the message, which is not usually the case for eukaryotic miRNAs (Gottesman, LDE225 2005). However, recently, our knowledge of the functions of sRNAs has been extended by demonstrations

that sRNAs can target not only the 5′ ends but also the 3′ ends, the internal part of RNAs, some combinations within the transcripts, and even proteins (see the reviews by Gottesman, 2005; Vogel & Wagner, 2007; Thomason & Storz, 2010). The functions of miRNAs have been extended also by the finding of miRNAs that bind to the promoter regions of DNA (Li et al., 2006; Schnall-Levin et al., 2010). Applying the uniform identification system RG7420 mw used for miRNAs – a precursor structure that contains the c. 22 nt miRNA sequence within one arm of the hairpin (Ambros et al., 2003) – we show that msRNAs surrounding the sequence fulfil this potential fold-back structure using the RNA-folding software and also code for miRNA*-like msRNA* sequences (see Fig. 2b for an example of the msRNA structure). Although deep sequencing and Northern blot data show the existence of a family of msRNAs, the possibility that many of them originated from randomly degraded larger forms of RNAs cannot be excluded. However, a single, clear, unsmeared band of msRNA-428 revealed by the Northern blot (Fig. 2c) suggests that at least some of them may be specifically processed from the longer RNAs rather than produced in the course of random degradation. In this case, msRNAs may have functional activity in bacteria.