Our findings suggest that rhein inhibits the invasion of NPC cells may be mediated in part through the suppression of MMP-9 and VEGF expression via the modulation of NF-kappa B signaling pathway. (C) 2008 Elsevier Ltd. All rights reserved.”
“Ultrasound-assisted alkaline pretreatment of sugarcane bagasse (SCB) for fermentable sugar production was carried out and the influence of particle size, liquid to solid ratio (LSR), NaOH concentration, temperature and sonication time on delignification and reducing
sugar production was ascertained with Placket-Burman design. The best combination see more of each significant factor was determined by a central composite design (CCD) and optimum pretreatment conditions for maximum reducing sugar yield (96.27%) were particle size of 0.27 mm, LSR of 25 ml/g, NaOH concentration of 2.89% (w/v), temperature of 70.15 degrees C and pretreatment time of 47.42 min. Under these conditions, 92.11% of theoretical reducing sugar yield was
observed experimentally. The substantial reduction in pretreatment time and temperature with improved efficiency is the most attractive features of the ultrasound-assisted BAY 57-1293 purchase alkaline pretreatment. (C) 2012 Elsevier Ltd. All rights reserved.”
“Here, we report on the application of five previously developed microsatellite markers (simple sequence repeats, SSRs) to monitor an isolate of the entomopathogenic fungus Beauveria bassiana (Bals.) Vuill. in different environments. Discriminatory power of these SSR markers was assessed in two commercialized B. bassiana isolates CA3 order as well as in 16 B. bassiana isolates from a world-wide collection, and three of the five SSR markers were estimated to allow a confident discrimination among the given isolates. Sensitivity thresholds of 0.1 pg DNA were subsequently determined for all SSR markers
in case pure genomic fungal B. bassiana DNA was used as a template for PCR assays, but threshold levels varied depending on the environment (soil, plant) of the PCR assay. Furthermore, presence of a commercialized B. bassiana isolate was monitored via these SSR markers in three different types of potting substrates over a period of 14 weeks. With two SSR markers, strain-specific products were detected up to 14 weeks after application of B. bassiana to the substrate. Infectivity of B. bassiana conidia in the respective soil samples was confirmed by the Galleria baiting technique. Together these results indicate that molecular markers like SSRs specific for commercialized strains of entomopathogenic fungi are important tools to monitor a particular fungal strain in complex environmental samples such as bulk soil or plant DNA. (C) 2013 Elsevier Inc. All rights reserved.