In the neoepidermis developed in vivo, the p63 nuclear antigen was expressed in basal cells and in a fraction of suprabasal cells. Protein p63 is a marker of selleck chem inhibitor progenitor and transit-amplifying cells that are essential for the proliferative potential in the stratified epithelia [18�C20]. This protein plays an important role in keratinocyte differentiation [20].Cells usually do not grow on skin covers. Exceptions are biological covers including collagens; however, the attachment and cell proliferation depend on the particular structure, purity, type of storing, type of sterilization, and so forth. Differentiation of keratinocytes takes place only on materials similar to the normal dermis, usually in the presence of mesenchymal cells. We have tested several types of synthetic wound covers (e.
g., Grassolind, Viacell, AskinaThinSite, Inadine, etc.) with negative results for cell growth. Limited cell growth was achieved on adhesive dressing Suprathel. Biopad was selected as comparative material for keratinocyte cultivation as it represents sponge-shaped (3D structured) pure collagen.The fact that keratinocytes cultured on XD organized themselves in a manner similar to that of normal skin, while those cultured on the equine collagen Biopad grew in a disorderly fashion, supports our assumption that the natural biological structure of the dermis plays an important role in formation of the epidermal architecture. Signals from the underlying mesoderm seem to be required to trigger the discrete functions of p63 and other proteins in the ectoderm that play a key role in keratinocyte commitment, differentiation, and regeneration [20].
Our results are in accordance with the results of Feng et al. [21] documenting that porcine acellular dermal matrix used to cover deep second-degree burns preserves residual dermal tissue and epithelium and helps to accelerate the regeneration of epithelial and stem cells, thus shortening the healing time, remodeling skin structure, and consequently preventing hypertrophic scars at inception. One shot therapy without dressing exchange avoids secondary injury to the wound [21].5. ConclusionThe firm natural structure of XD stimulates proliferation and differentiation of human primary keratinocytes. The keratinocytes cultured on XD organized themselves in a manner similar to that of normal skin, while those cultured on the equine collagen Biopad grew in a disorderly fashion.
These results support our assumption that the natural biological structure of the dermis plays an important role in wound epithelization and formation of the epidermal architecture. The healing effects of XD result from the support of proliferation, migration, and differentiation of the patient’s keratinocytes.DisclosureE. Batimastat Matouskova is an associated scientist of MEDICEM Institute. The rest of authors have nothing to disclose.