Assembly of L. crescens yielded a complete genome containing two predicted prophages. Sequence comparison of Candidatus L. asiaticus www.selleckchem.com/products/PF-2341066.html to L. crescens indicated that the species are 75.5% similar [11]. However, the prophage regions are not homologous. Sequencing and analysis of the L. crescens genome provided insight to the metabolic requirements of Candidatus L. asiaticus, which appears to lack the ability to synthesize thiamine and several essential amino acids. Less is known about the virulence of Candidatus L. asiaticus, although bacteriophages have also been known to add virulence to an otherwise non-pathogenic bacterium [54]. Further genomic analysis indicated that virulence in Candidatus L. asiaticus could also be due to a zinc ABC transporter. While the sequencing of L.

crescens gave much insight into the Liberibacter genus, further experiments must be conducted to verify these predictions. Notes Abbreviations: EMBL- European Molecular Biology Laboratory NCBI- National Center for Biotechnology Information (Bethesda, MD, USA), HLB- Huanglongbing, ZC- Zebra Chip, PBT- Papaya bunchy top, ICBR- Interdisciplinary Center for Biotechnology Research, RAST- Rapid Annotation using Subsystem Technology, NR- non-redundant, CDD- Conserved Domain Database, KEGG- Kyoto Encyclopedia of Genes and Genomes, KASS- automatic annotation server, ABC- ATP-binding cassette, Tat- twin-arginine translocation, flp- fimbrial low-molecular-weight protein
Brevibacterium senegalense strain JC43T (= CSUR P155 = DSM 25783) is the type strain of B. senegalense. sp. nov.

This bacterium is a non-motile, rod-shaped, Gram-positive, catalase-positive bacterium that was isolated from the stool of a healthy Senegalese patient as part of a study aiming at cultivating individually all bacterial species within human feces. Bacterial taxonomy has undergone many changes over recent years. The DNA-DNA hybridization and G+C content criteria, once considered as gold standards [1], were gradually replaced by gene sequencing. In particular, 16S rRNA sequencing has deeply changed the way bacteria and archaea are classified [2]. More recently, the development of high throughput genome sequencing methods and mass spectrometric analyses of bacteria have provided a wealth of genetic and proteomic information [3].

We recently used a polyphasic approach [4] that includes genomic data, MALDI-TOF spectrum and major phenotypic characteristics to describe new bacterial species [5-11]. The genus Brevibacterium (Breed 1953) [12] was created in 1953 to gather short non-spore-forming and non-branching rods. To date, this genus is comprised Carfilzomib of Gram-positive, irregular, rod-shaped, non-acid-fast bacteria, and contains 31 recognized species with validly published names [13]. Brevibacterium is the type genus of the family Brevibacteriaceae (Breed 1953) [14].