As surprising as it may appear, the presence of bacteria in the gut lumen contributes to the integrity of the intestinal epithelial barrier [26]. This is achieved by a series of molecular events induced

by the gut microbiocenosis. One event is increased synthesis of pIgR (epithelial polymeric immunoglobulin receptor), which provides the translocation of sIgA (secretory IgA) Selleck Cabozantinib from LP in the intestinal lumen [27] (Fig. 1). sIgA, a valuable local defence tool, prevents unwanted antigens from adhering to the intestinal mucosa. pIgR-deficient mice that lack sIgA and sIgM exhibit an altered barrier function of the intestinal epithelium, but are also more prone to gaining oral tolerance [28]. This argues for a dual function of a competent intestinal mucosa, ensuring both protection against harmful agents and acceptance of small amounts of certain antigens which induce the development of Tregs. Another event triggered by some species of commensal bacteria is the abrogation of polyubiquitination, necessary for IκB-α degradation [29]. IκB-α is the molecule that controls the activity of nuclear factor (NF)-κB, acting as its suppressor. IκB-α degradation is dependent on both phosphorilation and polyubiquitination. A longer life of IκB-α due to suppressed polyubiquitination will result in reduced MK-2206 research buy proinflammatory activity of NF-κB. The barrier function of the enterocytes is completed by anti-microbial peptides (AMP)

and mucin proteins production [30]. We must specify that AMPs are produced mainly by Paneth cells, and intestinal mucus is the major result of goblet cell activity. Enterocytes produce mucin proteins, which compose the glycocalix, and anti-microbial factors such as β-defensins and hepatocarcinoma–intestine–pancreas/pancreatitis-associated protein (HIP/PAP) [31]. β-defensins bind to the microbial cell membrane and, once embedded, form pore-like membrane defects that allow efflux of ions and nutrients. HIP/PAP is a member of the C-type lectin family and has a promising potential for ID-8 tissue regeneration and protection against apoptosis and cellular stress, being already tested as an agent for the therapy of acute

liver failure in humans [32]. Human β-defensin-1 (HBD-1) is expressed constitutively in enterocytes, while HBD-2 and HBD-3 are induced by microbial products and inflammatory cytokines [33,34]. Inducible expression of HBD-2 and HIP/PAP proteins in enterocytes was shown to be influenced by Toll-like receptor (TLR)- or myeloid differentiation primary response gene 88 (MyD88)-dependent signalling [35,36]. β-defensins may also chemoattract immature DCs [37] and have direct effects on DC function by inducing up-regulation of co-stimulatory molecules and DC maturation [38]. Enterocytes possess specialized receptors of the pathogen recognition receptors (PRR) family, such as TLRs and nucleotide oligomerization domain (NOD)-like receptors.