Algae grown in August under nutrient enrichment had significantly lower values of Chl a per unit biomass than those detected amongst all other treatment combinations (three-way factorial ANOVA, F(3,32) = 23.9, P < 0.0001; Table 2). Also, the interaction of Time and Scenario was explained by a decrease in Chl a concentrations for algae grown under the August-B1 treatment compared with all algae grown in November. However, in November, algae

kept under the B1 scenario contained more chlorophyll a than algae grown CX-4945 in vitro under August-A1FI conditions (three-way factorial ANOVA, F(3,32) = 3.6, P < 0.02, post hoc: AugE < all; B1Aug < Nov; A1FIAug < B1Nov). The combined concentration MK-8669 nmr of the xanthophylls antheraxanthin and violaxanthin normalized to Chl a (μgpigment · mgChla−1) was significantly affected by the interaction between Nutrients and Time (three-way factorial ANOVA, F(3,128) = 7.5, P < 0.01). This was due to an increase in the relative concentration of these xanthophylls in August under elevated nutrients compared with

all other treatment conditions. Zeaxanthin was not detected in these dark-adapted samples. β-carotene (μgpigment · gfw−1; Fig. 3) was generally at its lowest value in August (three-way factorial ANOVA, F(1,32) = 59.6, P < 0.0001). An interaction between Nutrients × Scenario was observed (three-way factorial ANOVA, F(3,32) = 3.2, P = 0.04), with post hoc analysis suggesting that β-carotene concentrations were higher for algae grown under A1FI, as opposed to PD scenarios, when in the presence of ambient nutrient concentrations. Nutrient concentrations within the algal tissue differed significantly between treatments. Carbon tissue concentrations involved a significant three-way interaction amongst the factors (carbon: three-way factorial ANOVA, F(3,32) = 3.5, P = 0.03, Fig. 4; Table 4). In both August and November, nutrient addition had a detrimental effect

medchemexpress on carbon tissue content irrespective of Scenario (three-way factorial ANOVA, F(3,32) = 86, P < 0.0001). In November, adding nutrients tended to have a detrimental effect that was more pronounced for PI and PD scenarios than for either B1 or A1FI scenario (two-way factorial ANOVA, F(3,16) = 5.4, P < 0.0001). Nitrogen and phosphorus tissue concentrations were elevated in algae grown in enriched nutrient environments (three-way factorial ANOVA, nitrogen: F(1,32) = 402, P < 0.0001, phosphorus: F(1,32) = 223, P < 0.0001; Fig. 4). Nitrogen, like carbon, concentrations, showed a complex three-way interaction (three-way factorial ANOVA, F(3,32) = 5.2, P = 0.005). In November, a significant Nutrient × Scenario interaction (two-way factorial ANOVA, F(3,16) = 6.9, P = 0.004) followed by post hoc analysis confirmed that higher nitrogen was stored in samples from nutrient enriched treatments.