A closer examination of individ ual proteins assigned to these GO terms revealed that trans 2 enoyl CoA, aminotrans ferases and ribosomal professional teins tended for being down regulated, whereas transcriptional activator Myb linked proteins, and signal transduction histidine kinase tended to get up regulated in symbiont bearing cells rela tive to symbiont free of charge cells. Determined by the knowledge of P. bursaria accumulated to date, functions could be inferred for many of the 6 higher est ranking GO terms. Down regulation of ribosomal proteins in symbiont bearing P. bursaria cells suggests cussed above, heat shock 70 kDa protein and glutathione S transferase genes were up regulated and down regulated as shown by the good and nega tive values of logFC, respectively, in symbiont bearing cells compared to symbiont no cost cells.

Of the ten,557 unigenes, more helpful hints eight have been annotated as Hsp70 with logFC of ?1. 3 to 5. 6, by using a median of 0. 92. Symbiont bearing P. bursaria cells are regarded to demonstrate a greater survival ratio against nickel chloride, high temperature, and hydro gen peroxide compared to the symbiont free cells show. In addition, P. caudatum cells reportedly obtain heat shock resistance by infection of endonucler symbiotic bacteria Holospora, and osmotic shock resistance. Hori and Fujishima reported that H. obtusa bearing paramecia expressed high levels of Hsp70 mRNA even at 25 C. The up regulation of the transcripts en coding Hsp70 could be associated on the hosts tolerance to environmental fluctuations. With the 10,557 transcripts, seven were annotated as GST and tended to get down regu lated with logFC of ?5.

seven to ?0. 12, having a median of ?0. 85. This enzyme is related to safety of cells from oxidative anxiety, as proven by McCord and Fridovich and by Veal et al. Although it was conceivable that photograph oxidative pressure in symbiont bearing P. bur saria cells is higher than that in symbiont absolutely free ones, our data showed opposite final results through the prediction. A simi lar end result was obtained by H?rtnagl read this article and Sommaruga. They advised that the presence of algal symbionts mini mizes photo oxidative strain. Consequently, unique expression ranges in these genes involving symbiont absolutely free and symbiont bearing P. bursaria agree very well with vary ences in cytological phenomena observed in these para mecia, suggesting that these proteins appear for being associated with the symbiosis.

Immunological detections on the gene merchandise and comparisons of the amount of the antigens or qualitative PCR among the symbiont no cost and also the symbiont bearing paramecia are essential for long term expe riments.