855, p < 0.01). Computerized NCTs was able to diagnose
www.selleckchem.com/products/Adrucil(Fluorouracil).html MHE with 88.9% sensitivity and 87.5% specificity (Area under the curve = 0.958, p < 0.01). Conclusion: Computerized Number Connection Tests is established and preliminarily confirmed to be a valid and reliable method for screening of MHE. Key Word(s): 1. liver cirrhosis; 2. MHE; 3. NCT; Presenting Author: CHAO DU Additional Authors: DEMING JIANG Corresponding Author: CHAO DU Affiliations: Chengdu Military Command Objective: To explore the possibility and validity of differentiation of rat bone mesenchymal stem cells (BMSCs) into hepatocytes with a culture system containing salidroside and cholestatic rat serum in vitro. Methods: BMSCs were isolated by selleck compound plastic adherence from whole bone marrow of health SD rat at the age of 2–3 weeks, identifying
stem cell surface markers of CD45, CD14, CD34, CD79a, CD90, CD105 by the flow assay; cholestatic serum were prepared by common bile duct ligation from 10 health SD rats at the age of 10–13 weeks. the 3rd – passage BMSCs were divided into three groups for vitro induction by the different culture systems : Group A : basic growth medium plus 5% cholestatic serum; Group B : basic growth medium plus 5% cholestatic serum plus 30UM salidroside; group C : basic growth medium plus 5% cholestatic serum plus 20 ug / L Hepatocyte Growth Factor (HGF); observing changes of cell morphology during culture time in each group-induced, RT-PCR assay to detect mRNA expression of alpha-fetoprotein (AFP) and albumin (ALB); Western-Blot assay to detect protein expression levels of AFP and ALB. Results: The BMSCs highly express CD90, CD105, did not express CD45, CD14, CD34, CD79a, the cells of three groups appear polygonal and binucleate cells in the procedure of induction; The mRNA and protein expression of AFP and ALB emerged in the three groups on the 7th day; in the same period the lowest expression
ratio was in group A (p < 0.05), while there was no significant difference between group B and group C (p > 0.05). Conclusion: Salidroside and cholestatic serum can effectively induce BMSCs differentiated into hepatocytes. Key Word(s): 1. cholestatic serum; 2. salidroside; 3. induction; 4. HGF; Arachidonate 15-lipoxygenase Presenting Author: SU SHUAI Additional Authors: LIUWEN TIAN, WANGBANG MAO Corresponding Author: SU SHUAI Affiliations: TIANJIN MEIDICAL UNIVERSITY GENERAL HOSPITAL Objective: To investigate the clinical characteristics of Chinese patients with Peliosis hepatis. Methods: ReIevant data of Chinese Peliosis hepatis Patients were retrieved from PubMded and CNKI database and a meta–analysis was conducted Results: A total of 27 Peliosis hepatic cases had been reported by Chinese hospitals with obscure causes, mainly manifestied as abdominal distention and edema, accompanied with transudative ascites and maybe Hemorrhagic ascites. Liver rupture was reported in 4 patients and Liver dysfunction was in 44.44% patients..