According to Marfo GSK1120212 mw et al., 35% of the patients on the waiting list are sensitized with PRA levels > 0%, and 15% are highly sensitized with PRA levels > 80% [1]. In some regions of the United States, the waiting time on the transplant list can exceed five years and due to organ shortage, this scenario is not changing in the near future. It has been thoroughly described that highly sensitized patients have longer waiting times and some may never receive a transplant [1]. In Mexico, roughly 75% of renal transplants are from living donors and approximately 2300 kidney transplants per year have been performed during the last 3 years [8]. Although

there has been a decrease in the mortality rate of patients on dialysis, approximately 15 to 20% still die each year, which emphasizes the importance of early transplantation [4] and [9]. There is an evident financial cost and emotional burden secondary to maintaining

a highly sensitized patient on dialysis in comparison to early transplantation. The impact of kidney MS-275 transplantation on morbidity, mortality, quality of life and medical expenses is undeniable. The main objective of this study was to determine the probability of patients in the deceased donor (DD) waiting list at the National Institute of Medical Sciences and Nutrition (INCMNSZ) in Mexico City to receive a kidney transplant (KT), based on the degree of sensitization determined by % PRA. Acute rejection rate, graft function, patient and graft survival, and causes for patient death/graft loss were also analyzed. This protocol was approved by the Institutional Committee of Medical Ethics and performed in accordance with the revised Declaration of Helsinki content and Good Clinical Practice Guidelines. The renal transplant DD waiting list database was reviewed from January 2005 to August 2012 at the Histocompatibility Laboratory

at INCMNSZ. For each DD event, we documented the donor’s demographic characteristics (age and gender), donor’s blood group (ABO group), the number and ABO group of all the potential recipients considered, the results of the lymphocyte cross-match test [CxM (AHG-CDC)] for each potential recipient considered, Megestrol Acetate the % PRA of each potential recipient (highest % PRA documented in the last three determinations) and which patients consequentially received a DD kidney transplant. Anti-HLA antibodies were tested by the Luminex technique using test kits purchased from One Lambda, Inc., Canoga Park, CA. In the patients on the waiting list, a LabScreen Mixed Classes I & II and a LabScreen PRA Classes I & II were simultaneously performed. Only those with positive results in either test received a Labscreen Single Antigen test. When available, the result of pre-transplant DSA assessment using the LABScreen Single Antigen Classes I & II was gathered for the analysis.