In the untrained group, the contributions of CHO and fat to total

In the untrained group, the contributions of CHO and fat to total EE during exercise were lower and higher, respectively, after the CAJ supplementation than after taking the PLA supplementation (80 vs 90%; p< 0.05 and 20 vs 10%; p< 0.05) (Figure 2). In the trained group, the contributions of CHO and

fat to total EE during exercise were also lower and higher, respectively, after CAJ supplementation than after taking the AZD1480 solubility dmso PLA (73 vs 89%; p<0.05 and 27 vs 11%; p<0.05) (Figure 2). Figure 1 CHO (A) and fat (B) oxidation rates during exercise at 85% or after 4-week placebo (PLA) and cashew apple juice (CAJ) supplementation. Values are mean ± SE, n = 10 in each group. CHO, carbohydrate. * Nutlin-3a cost significantly different from before supplementation, p<0.05, # significantly different from the PLA group, p<0.05, α significantly different from the untrained group, p<0.05. Figure 2 Relative contribution of substrate to total energy expenditure during exercise at 85% or after 4-week placebo (PLA) and cashew apple juice (CAJ) supplementation. Values are mean, n = 10 in each group. * Significantly different from before supplementation, p<0.05, # significantly different PCI-32765 in vitro from the PLA group, p<0.05, α significantly different from the untrained group, p<0.05. In both the trained and untrained groups, resting

plasma vitamin C concentrations were significantly increased after the CAJ supplementation (p<0.05) without any change after receiving the PLA (Figure 3). There were significantly

higher vitamin C concentrations after AMP deaminase the CAJ supplementation than the PLA administration (p<0.05). CAJ supplementation, however, had no effect on the metabolic profiles taken at rest and after exercise sessions, including serum glucose, insulin, TC, TG, HDL, or LDL, in either the trained or untrained subjects. With the PLA administration, there were also no significant changes in any parameters over the 4-week treatment period in either the trained or untrained subjects. Figure 3 Plasma vitamin C concentration immediately after exercise at 85% or after 4-week placebo (PLA) and cashew apple juice (CAJ) supplementation. Values are mean ± SE, n = 10 in each group. * Significantly different from before supplementation, p<0.05, # significantly different from the PLA group, p<0.05, α significantly different from the untrained group, p<0.05. Discussion This study showed that the 4-week CAJ supplementation increased fat contribution and decreased CHO contribution to total energy expenditure during high-intensity exercise in both the trained and untrained subjects, with a greater change in the trained subjects. It should be noted that this study assessed whole-body substrate utilization. Therefore, the changes in specific sources of energy used cannot be defined.

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